GOJI-BEERE
Aktuelle wissenschaftliche Studien | 15-30
15: Yakugaku Zasshi. 2007 Oct;127(10):1715-21.
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Hypoglycemic effects and mechanisms of action of Cortex Lycii Radicis on alloxan-induced diabetic mice.
Gao D, Li Q, Liu Z, Li Y, Liu Z, Fan Y, Li K, Han Z, Li J.
Department of Biological Engineering, College of Environmental and Chemical Engineering, Yanshan University, No. 438 Hebei Street, Qinhuangdao 066004, P.R. China.
Cortex Lycii Radicis (CLR) has been used as a traditional Oriental medicine as an antipyretic and to treat pneumonia, night-sweats, cough, hematemesis, inflammation, and diabetes mellitus for centuries. This study aimed to determine the effects of CLR on alloxan-induced diabetic mice and its mechanisms. Based on thin-layer chromatography (TLC) assay, the main compounds of CLR include an organic acid, flavone, alkaloid, polysaccharide, anthraquinone, and saponin. The mice were divided into four groups: normal control (NC), diabetes control (DC), diabetes+high-dose CLR (200 mg kg(-1)), and diabetes+low-dose CLR (100 mg kg(-1)). The diabetic mice were administered CLR daily for 28 days. The CLR treatment resulted in significant decreases in fasting blood glucose, total cholesterol, and triglycerides. CLR also showed a tendency to improve body weight gain in diabetic mice. Furthermore, the serum insulin level of each group was assayed, and the DC group had a lower serum insulin level than the NC group. Insulin levels were dose dependently raised in the CLR-treated groups compared with the DC group. According to single-cell gel electrophoresis and LD(50) analysis, CLR was nontoxic to the animals. The results indicate that CLR alleviates the blood glucose and lipid increases associated with diabetes and improves the abnormal glucose metabolism and increases insulin secretion by restoring impaired pancrease beta-cells in alloxan-induced diabetic mice. The results suggest that CLR has hypoglycemic potential and could be useful in diabetes therapy.
Publication Types:
PMID: 17917429 [PubMed - indexed for MEDLINE]
16: J Soc Integr Oncol. 2007 Summer;5(3):130.
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Lycium barbarum.
[No authors listed]
PMID: 17761133 [PubMed - indexed for MEDLINE]
17: Phytother Res. 2008 Feb;22(2):190-6.
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Protective effects of an herbal formulation of Radix Astragali, Radix Codonopsis and Cortex Lycii on streptozotocin-induced apoptosis in pancreatic beta-cells: an implication for its treatment of diabetes mellitus.
Chan JY, Leung PC, Che CT, Fung KP.
Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China.
Hyperglycemia is one of the main causes of oxidative stress in type 2 diabetes mellitus. During hyperglycemia, the increased level of various reducing sugars in the blood enhances the production of reactive oxygen species (ROS) and triggers tissue damage, especially in pancreatic beta-cells. Streptozotocin (STZ) is a diabetogen that causes diabetes mellitus via ROS-induced apoptosis in beta-cells. In this study, SR10, an herbal formulation consisting of the aqueous extracts of Radix Astragali, Radix Codonopsis and Cortex Lycii was examined for its antidiabetic effects in vitro. SR10 treatment resulted in significant enhancement of survival rate of rat pancreatic beta-cells which were treated by streptozotocin. SR10 apparently reduced apoptosis of streptozotocin-treated beta-cells by decreasing DNA fragmentation, sub-G(1) peak area and percentage of apoptotic cells. Nitric oxide (NO) production in streptozotocin-treated cells was inhibited by SR10 via the suppression of the expression of inducible nitric oxide synthase (iNOS). The implication of SR10 in treating type 2 diabetes mellitus was discussed.
Publication Types:
PMID: 17726733 [PubMed - indexed for MEDLINE]
18: Cell Mol Neurobiol. 2008 Aug;28(5):643-52. Epub 2007 Aug 21.
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Use of Anti-aging Herbal Medicine, Lycium barbarum, Against Aging-associated Diseases. What Do We Know So Far?
Chang RC, So KF.
Laboratory of Neurodegenerative Diseases, Department of Anatomy, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China, rccchang@hkucc.hku.hk.
Lycium barbarum (Gouqizi, Fructus Lycii, Wolfberry) is well known for nourishing the liver, and in turn, improving the eyesight. However, many people have forgotten its anti-aging properties. Valuable components of L. barbarum are not limited to its colored components containing zeaxanthin and carotene, but include the polysaccharides and small molecules such as betaine, cerebroside, beta-sitosterol, p-coumaric, and various vitamins. Despite the fact that L. barbarum has been used for centuries, its beneficial effects to our bodies have not been comprehensively studied with modern technology to unravel its therapeutic effects at the biochemical level. Recently, our laboratory has demonstrated its neuroprotective effects to counter neuronal loss in neurodegenerative diseases. Polysaccharides extracted from L. barbarum can protect neurons against beta-amyloid peptide toxicity in neuronal cell cultures, and retinal ganglion cells in an experimental model of glaucoma. We have even isolated the active component of polysaccharide which can attenuate stress kinases and pro-apoptotic signaling pathways. We have accumulated scientific evidence for its anti-aging effects that should be highlighted for modern preventive medicine. This review is to provide background information and a new direction of study for the anti-aging properties of L. barbarum. We hope that new findings for L. barbarum will pave a new avenue for the use of Chinese medicine in modern evidence-based medicine.
PMID: 17710531 [PubMed - in process]
19: Phytother Res. 2007 Nov;21(11):1020-4.
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Protective effect of Lycium barbarum on doxorubicin-induced cardiotoxicity.
Xin YF, Zhou GL, Deng ZY, Chen YX, Wu YG, Xu PS, Xuan YX.
State Key Laboratory of Safety Evaluation for New Drug, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China.
The objective of this work was to explore the hypothesis that Lycium barbarum (LB) may be protective against doxorubicin (DOX)-induced cardiotoxicity through antioxidant-mediated mechanisms. Male SD rats were treated with distilled water or a water extract of LB (25 mg/kg, p.o.) daily and saline or DOX (5 mg/kg, i.v.) weekly for 3 weeks. Mortality, general condition and body weight were observed during the experiment. DOX-induced cardiotoxicity was assessed by electrocardiograph, heart antioxidant activity, serum levels of creatine kinase (CK) and aspartate aminotransferase (AST) and histopathological change. The DOX group showed higher mortality (38%) and worse physical characterization. Moreover, DOX caused myocardial injury manifested by arrhythmias and conduction abnormalities in ECG (increased QT and ST intervals and ST elevation), a decrease of heart antioxidant activity, an increase of serum CK and AST, as well as myocardial lesions. Pretreatment with LB significantly prevented the loss of myofibrils and improved the heart function of the DOX-treated rats as evidenced from lower mortality (13%), normalization of antioxidative activity and serum AST and CK, as well as improving arrhythmias and conduction abnormalities. These results suggested that LB elicited a typical cardioprotective effect on DOX-related oxidative stress. Furthermore, in vitro cytotoxic study showed the antitumor activity of DOX was not compromised by LB. It is possible that LB could be used as a useful adjunct in combination with DOX chemotherapy. Copyright (c) 2007 John Wiley & Sons, Ltd.
Publication Types:
PMID: 17622973 [PubMed - indexed for MEDLINE]
20: Int J Mol Med. 2007 Aug;20(2):261-8.
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Characterization of the effects of anti-aging medicine Fructus lycii on beta-amyloid peptide neurotoxicity.
Yu MS, Lai CS, Ho YS, Zee SY, So KF, Yuen WH, Chang RC.
Laboratory of Neurodegenerative Diseases, Department of Anatomy, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.
Alzheimer's disease (AD) is an age-related neurodegenerative disease. There are increasing lines of evidence showing that the molecular signaling pathways in aged cells are altered so that cells are susceptible to injury. We and other laboratories have demonstrated the significant involvement of double-stranded RNA-dependent protein kinase (PKR) in beta-amyloid (A beta) peptide neurotoxicity and in AD. Fructus lycii (the fruit of Lycium barbarum) has long been used in oriental medicine as an anti-aging agent. Our previous studies demonstrated that the aqueous extract isolated from L. barbarum exhibited significant protection on cultured neurons against harmful chemical toxins such as A beta and dithiothreitol. We also showed that the polysaccharide-containing extract (LBP) from L. barbarum exhibited neuroprotective effects in the retina against ocular hypertension in a laser-induced glaucoma animal model. In this study, we aimed to investigate whether LBP can elicit neuroprotection to neurons stressed by A beta peptides. Furthermore, we planned to isolate and identify the neuroprotective agent from LBP using chromatographic methods. Our results showed that pretreatment of LBP effectively protected neurons against A beta-induced apoptosis by reducing the activity of both caspase-3 and -2, but not caspase-8 and -9. A new arabinogalactan-protein (LBP-III) was isolated from LBP and attenuated A beta peptide-activated caspase-3-like activity. LBP-III markedly reduced the phosphorylation of PKR triggered by A beta peptide. Since the phosphorylation state of PKR increased with age, reduction of its phosphorylation triggered by A beta peptide may implicate that LBP-III from Fructus lycii is a potential neuroprotective agent in AD. As herbal medicine has received increasing attention for the treatment of AD, our study will open a window for the development of a neuroprotective agent for anti-aging from Chinese medicine.
Publication Types:
PMID: 17611646 [PubMed - indexed for MEDLINE]
21: Fitoterapia. 2007 Dec;78(7-8):574-6. Epub 2007 May 24.
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Antimicrobial activities of Indian Berberis species.
Singh M, Srivastava S, Rawat AK.
Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow-226001, India.
The antimicrobial activity of hydroalcoholic extracts of four Berberis species viz. Berberis aristata, Berberis asiatica, Berberis chitria and Berberis lycium were tested against eleven bacterial and eight fungal strains. B. aristata root extract gave low MICs values against Bacillus cereus, Escherichia coli, Staphylococcus aureus and Aspergillus flavus while stem extract against B. cereus and Streptococcus pneumoniae.
PMID: 17583443 [PubMed - indexed for MEDLINE]
22: Brain Res. 2007 Jul 16;1158:123-34. Epub 2007 May 6.
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Characterizing the neuroprotective effects of alkaline extract of Lycium barbarum on beta-amyloid peptide neurotoxicity.
Ho YS, Yu MS, Lai CS, So KF, Yuen WH, Chang RC.
Laboratory of Neurodegenerative Diseases, Department of Anatomy, The University of Hong Kong, Pokfulam, Hong Kong SAR.
Lycium barbarum is an oriental medicinal herb that has long been used for its anti-aging and cell-protective properties. Previous studies have shown that aqueous extracts from L. barbarum exhibit neuroprotection via inhibiting pro-apoptotic signaling pathways. Other active components can also be accomplished by novel alkaline extraction method, which may give different profiles of water-soluble components. We hypothesize that another active component obtained by alkaline extraction method exerts different biological mechanisms to protect neurons. In this study, we aim to examine the neuroprotective effects from the alkaline extract of L. barbarum, namely LBB, to attenuate beta-amyloid (Abeta) peptide neurotoxicity. Primary cortical neurons were exposed to Abeta-peptides inducing apoptosis and neuronal cell death. Pretreatment of LBB significantly reduced the level of lactate dehydrogenase (LDH) release and the activity of caspase-3 triggered by Abeta. "Wash-out" procedures did not reduce its neuroprotective effects, suggesting that LBB may not bind directly to Abeta. We have further isolated three subfractions from LBB, namely LBB-0, LBB-I and LBB-II. LBB-I and LBB-II showed differential neuroprotective effects. Western blot analysis demonstrated that LBB-I and LBB-II markedly enhanced the phosphorylation of Akt. Taken together, our results suggested that the glycoconjugate isolated from novel alkaline extraction method can open up a new avenue for drug discovery in neurodegenerative diseases.
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PMID: 17568570 [PubMed - indexed for MEDLINE]
23: Anim Reprod Sci. 2008 Jun;106(1-2):25-35. Epub 2007 Mar 30.
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Effect of sugars on maturation rate of vitrified-thawed immature porcine oocytes.
Huang J, Li Q, Zhao R, Li W, Han Z, Chen X, Xiao B, Wu S, Jiang Z, Hu J, Liu L.
College of Animal Science, Northwest Agriculture and Forestry University, Yangling, Shannxi Province 712100, China.
This study examined the effects of monosaccharide (glucose), disaccharide (sucrose) and polysaccharides (Ficoll and Lycium barbarum polysaccharide (LBP)) at different concentrations, using ethylene glycol (EG) as membrane-permeating cryoprotectant, on in vitro maturation of vitrified-thawed immature (GV) porcine oocytes. A total of 1145 oocytes were obtained by follicle aspiration from 496 ovaries of pigs slaughtered at a local abattoir and vitrified using a five-step method. After thawing and removal of cryoprotectant, oocytes were cultured for 44 h at 39 degrees C in a humidified atmosphere of 5% CO(2) in air. Oocytes were stained with DAPI and nuclear maturation was examined. The highest maturation rates were obtained in 1.5M glucose (8.62%), 0.75 M sucrose (20.0%), 3.0 g/ml Ficoll (13.79%) and 0.10 g/ml LBP (20.69%), respectively. The maturation rate using 0.75 M sucrose or 0.10 g/ml LBP was significantly higher compared to 1.5M glucose (P<0.05), but there was no significant difference from using 3.0 g/ml Ficoll (P>0.05). The percentage of oocytes reaching metaphase II (MII) stage in the cryopreserved groups was significantly lower than control (P<0.05). These results suggest that LBP is an effective non-permeating membrane cryoprotectant and 0.75 M sucrose or 0.10 g/ml LBP can be used as the vitrification solution for immature porcine oocytes.
Publication Types:
PMID: 17478061 [PubMed - indexed for MEDLINE]
24: Zhonghua Gan Zang Bing Za Zhi. 2007 Mar;15(3):204-8.
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[A study on the preventive effect of Lycium barbarum polysaccharide on the development of alcoholic fatty liver in rats and its possible mechanisms]
[Article in Chinese]
Gu S, Wang PL, Jiang R.
Department of Gastroenterology, First Affilated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China. saigu@20042004@yahoo.com
OBJECTIVE: To study the preventive effects of Lycium barbarum polysaccharide (LBP) on the development of alcoholic fatty liver (AFL) in rats and its possible mechanisms. METHODS: One hundred twenty-five Wister rats were randomly divided into 4 groups: a blank control group, with distilled water intragastric infusion (GI); an alcohol group, with alcohol GI; a 5% LBP plus alcohol GI group; and a 10% LBP plus alcohol GI group. Liver pathologic changes were studied together with the activity of serum ALT, AST, GGT, the activity of liver SOD, GSH-PX and the content of liver MDA, GSH, H2O2; CYP2E1 gene and protein expressions were also detected. RESULTS: At the end of ten weeks, the activity of serum AST [(132.3+/-25.7) U/L, (127.5+/-29.1)U/L] and GGT [(1.9+/-0.5)U/L, (1.8+/-0.7)U/L] of the two LBP groups were all significantly lower than those of the alcohol group [serum AST (245.7+/-32.1) and GGT (4.4+/-0.6)]. At the end of ten weeks, the content of liver MDA [(5.1+/-0.3) nmol/mg, (5.1+/-0.4) nmol/mg] and H2O2 [(135.4+/-23.5) mmol/g, (132.6+/-31.8) mmol/g] of the two LBP groups were significantly lower than those of the alcohol group [MDA (14.5+/-3.2) nmol/mgprot) and H2O2 (328.5+/-45.6)]. The activity of SOD [(206.7+/-13.2)U/L, (203.2+/-18.8)U/L], GSH-PX [(13.5+/-1.4)U/mg/min, (13.6+/-1.5)U/mg/min] and the content of GSH [(65.1+/-11.0)mg/g, (66.6+/-11.1) mg/g] of the two LBP groups were all significantly higher than those of the alcohol group [SOD (116.5+/-13.6)U/mg/min, GSH-PX (7.2+/-1.6)U/mg/min and the content of GSH (30.5+/-10.7)mg/g] (P less than 0.01). At the end of five weeks, levels of CYP2E1 gene and protein expression of the two LBP groups were 0.39+/-0.04, 0.40+/-0.06 and 3.49+/-0.36, 3.29+/-0.30 respectively. At the end of ten weeks, levels of CYP2E1 gene and protein expression of the two LBP groups were 0.41+/-0.05, 0.42+/-0.08 and 3.58+/-0.30, 3.36+/-0.37 respectively. They were all significantly lower than those of the alcohol group [the gene expression (5 week: 0.74+/-0.05, 10 week: 1.02+/-0.08) and the protein expression (5 week: 5.63+/-0.44, 10 week: 7.90+/-0.26)]. There were no typical alcoholic fatty liver pathologic changes observed in the two LBP groups. CONCLUSION: LBP can effectively prevent AFL. This may be due to its effects in inhibiting the hepatocyte CYP2E1 expression and prevention of lipid peroxidation.
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PMID: 17407712 [PubMed - in process]
25: Bioresour Technol. 2008 Mar;99(5):1383-93. Epub 2007 Mar 23.
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Effects of Chinese wolfberry (Lycium chinense P. Mill.) leaf hydrolysates on the growth of Pediococcus acidilactici.
Yeh YC, Hahm TS, Sabliov CM, Lo YM.
Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, United States.
Growth stimulating effects of LYCH leaf hydrolysates on Pediococcus acidilactici IMT101 cells were observed when MRS broth was supplemented with 20% (v/v) H1+H2, the mixture of hydrolysates prepared by a traditional tea-making process. Cells grown on MRS containing H1+H2 showed a shortened lag phase while yielding a cell concentration (X(s)) significantly higher than other conditions investigated entering stationary phase. The maximal specific growth rate (mu(max)) was also the highest among all. Microwave-assisted extraction (MAE) at 80 degrees C for 2h (M80(2h)) released more amino acids but less sugar (fructose, glucose, and sucrose) than in H1+H2. Both X(s) and mu(max) reached in M80(2h)-supplemented MRS broth were lower than those in MRS containing H1+H2. No correlations between amino acids and cell growth were found. P. acidilactici cells grown in MRS broth in general showed higher consumption of carbohydrate in comparison with those in M17 broth containing the same carbohydrate. In the absence of FOS, the increased glucose concentration in MRS when supplemented by H1+H2 hydrolysates appeared to be responsible for the stimulatory effects on P. acidilactici growth. The growth-enhancing effects of LYCH leaf hydrolysates indicate the potential of developing new applications for LYCH leaves in promoting the growth of other probiotic cells using a simple process.
PMID: 17383176 [PubMed - indexed for MEDLINE]
26: J AOAC Int. 2007 Jan-Feb;90(1):271-6.
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Determination of organophosphorus pesticides in Lycium barbarum by gas chromatography with flame photometric detection.
Li L, Liu F, Qian C, Jiang S, Zhou Z, Pan C.
China Agricultural University, College of Science, Department of Applied Chemistry, 100094, Beijing, People's Republic of China.
An analytical method was developed to determine 14 organophosphorus pesticide residues in Lycium barbarum, which is both a botanical medicine and a food. A 5 g sample is mixed with 10 mL ethyl acetate and, after shaking and centrifuging, 5 mL of the upper layer is removed, concentrated, and analyzed by gas chromatography (GC) with flame photometric detection. The essential feature of this method is that, for the purpose of reducing the burden of the GC system, 0.01 g activated carbon is used to absorb pigments during the cleanup procedure. Average recoveries of 14 organophosphorus pesticides added at 0.05, 0.1, and 0.5 mg/kg were 66.84-102.42, 71.07-97.93, and 62.50-96.24%, respectively. Limits of detection ranged from 5 to 15 microg/kg. The identities of the 14 pesticides were confirmed by GC/mass spectrometry detection in the selected-ion monitoring mode. This method is sensitive, simple, rapid, inexpensive, and safe.
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PMID: 17373460 [PubMed - indexed for MEDLINE]
27: Fen Zi Xi Bao Sheng Wu Xue Bao. 2006 Dec;39(6):516-26.
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[Calcium distribution in the developing anther of Lycium barbarurn L]
[Article in Chinese]
Yang SJ, Zhang YN, Ye L, Song YX, Tian HQ.
The Cchool of Life Science, Xiamen University, Xiamen.
We used potassium antimonate to precipitate "exchangeable cellular Ca2+"-calcium that is sufficiently loosely bound to combine with antimonite, to investigate the feature of calcium distribution during anther development of Lycium barbarurn L. Before the stage of microspore mother cell, few calcium-induced precipitates were found in sporogenous cells and the somatic cells of anther wall. When microspore mother cell (MMC) preparing meiosis, calcium precipitates appeared in the cytoplasm of tapetal cells and callus wall surrounding MMC. After the meiosis of MMC,abundant calcium precipitates were accumulated in the cytoplasm of early microspores,and then in pollen wall, especially in the part of germ-pores. During the late microspore stage,a big vacuole formed and the nucleus was forced to move to peripheral region. Calcium precipitates decreased sharply and might dissolve in the large vacuole. After microspore mitosis, calcium precipitates appeared in the big vacuole of 2-cellular pollen,and then the vacuole disappeared. After that, the calcium precipitates again appeared in the cytoplasm of 2-cellular pollen, and the cytoplasm became densely and storage materials like starches accumulated inside the pollen grains. When pollen maturating,many small calcium precipitates distributed in its cytoplasm,especially in nucleus. The feature of calcium distribution in the anther of Lycium barbarurn L. means that it plays some biological roles during microspore development.
Publication Types:
PMID: 17348204 [PubMed - in process]
28: Zhonghua Nan Ke Xue. 2007 Jan;13(1):82-6.
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[Effects of Fructus lycii and Radix astragali on the function of sertoli cells in rat testes]
[Article in Chinese]
Zhao LP, Xu Z, Zhang M, Sun HC, Tang F.
Department of Pathology, Bethune International Peace Hospital, Shijiazhuang, Hebei 050082, China. zlpb2003@163.com
OBJECTIVE: To investigate the effects of the Chinese herbal medicines Fructus Lycii and Radix Astragali on the function of the Sertoli cells in the rat testis and their mechanisms. METHODS: Sertoli cells from the testes of the SD rats aged 18 - 22 days were isolated and cultured. The effects of Fructus Lycii, Radix Astragali and the combined administration of the two on the proliferation of Sertoli cells in vitro were detected by MTT assay, and their effects on the level of INHbetaB mRNA transcription in Sertoli cells in vitro were investigated in both normal environment and peroxide-damaging environment by RT-PCR. RESULTS: The proliferation of Sertoli cells was promoted by either Fructus Lycii or Radix Astragali at high concentration (P < 0.05), and significantly promoted by the combined administration at high concentration (P <0.01). Sertoli cell INHbetaB transcription was significantly up-regulated by Fructus Lycii, Radix Astragali and their combined administration in vitro (P < 0.01). When the level of INHbetaB mRNA in Sertoli cells significantly dropped (P < 0.01) in the presence of injury induced by peroxide (H2O2), it could be elevated by Radix Astragali (P < 0.05) and significantly up-regulated by Fructus Lycii or the combined administration in vitro (P < 0.01). CONCLUSION: Fructus Lycii, Radix Astragali and the combined administration of the two could promote and protect INHbetaB mRNA in Sertoli cells in vitro.
Publication Types:
PMID: 17302041 [PubMed - indexed for MEDLINE]
29: Cell Biol Int. 2007 Jun;31(6):615-9. Epub 2006 Dec 30.
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Lycium barbarum polysaccharides regulate phenotypic and functional maturation of murine dendritic cells.
Zhu J, Zhao LH, Zhao XP, Chen Z.
Department of Biochemistry and Molecular Biology, College of Medicine, Zhejiang University, Hangzhou 310006, Republic of China.
Lycium barbarum polysaccharides (LBPs) have been known to have a variety of immunomodulatory functions including activation of T cells, B cells and NK cells. Dendritic cells (DC) are potent antigen-presenting cells that play pivotal roles in the initiation of the primary immune response. However, little is known about the immunomodulatory effects of LBPs on murine bone marrow derived dendritic cells (BMDC). In the present study, the effects of LBPs on the phenotypic and functional maturation of murine BMDC were investigated in vitro. Compared to the BMDC that were only subjected to treatment with RPMI1640, the co-expression of I-A/I-E, CD11c and secretion of IL-12 p40 by BMDC stimulated with LBPs (100 microg/ml) were increased. In addition, the endocytosis of FITC-dextran by LBPs-treated BMDC (100 microg/ml) was impaired, whereas the activation of proliferation of allogenic lymphocytes by BMDC was enhanced. Our results strongly suggest that LBPs are capable of promoting both the phenotypic and functional maturation of murine BMDC in vitro.
PMID: 17289406 [PubMed - indexed for MEDLINE]
30: Zhongguo Zhong Yao Za Zhi. 2006 Nov;31(22):1862-5.
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[Formulation and process optimization of formula Qiqi pellets prepared by extrusion-spheronization]
[Article in Chinese]
Chen HX, Jia XB, Chen Y, Pan GL, Cheng XD.
School of Pharmacy, Jiangsu University, Zhenjiang 212001, China. chhx2005@sohu.com
OBJECTIVE:To prepare traditional Chinese medicine formula Qiqi pellets by extrusion-spheronization and study the optimal formulation and process. METHOD: Qiqi pellets were prepared by a new style extrusion-spheronization equipment, the optimal formulation and process was obtained by the studies of influenitial factors and L9 (3(4)) orthogonal design, the micromeritic properties and product yield of pellets were determined. RESULT: The formula Qiqi pellets prepared by extrusion-spheronization were all spheral with smooth surface; the product yield was high. CONCLUSION: Extrusion-spheronization was suitable to produce Chinese Herbal Medicine pellets. The preparation process was simple and feasible; The quality of the prepared pellets was excellent. It was worth further study.
Publication Types:
PMID: 17285984 [PubMed - indexed for MEDLINE]
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