GOJI-BEERE
Aktuelle wissenschaftliche Studien | 76-95
76: Zhong Yao Cai. 2004 Aug;27(8):599-600.
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[The study on the effect of LBP on cleaning hydroxygen free radical by EPR technique]
[Article in Chinese]
Ni H, Qing D, Kaisa S, Lu J.
Xinjiang Institute of Medical and Minorities Materia Medical, Urumqi.
OBJECTIVE: To study the effect of Lycium barbarum polysaccharides (LBP) on cleaning hydroxygen free radical. METHODS: EPR. RESULT: When the concentrations of LBP were 0.25 mg/ml and 1.0 mg/ml, the cleaning rates of LBP to hydroxygen free radical were 18.64% and 87.29%. CONCLUSION: LBP in low concentration can obviously clean hydroxygen free radical, but its clearance ability in high concentration descends.
Publication Types:
PMID: 15658825 [PubMed - indexed for MEDLINE]
77: J Huazhong Univ Sci Technolog Med Sci. 2004;24(5):518-20, 527.
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Experimental study on the enhancement of murine splenic lymphocyte proliferation by Lycium barbarum glycopeptide.
Du G, Liu L, Fang J.
Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
In order to investigate the immunoactivity of Lycium Barbarum glycopeptide (LBG), the routinely prepared murine splenic lymphocyte suspension was separately added into the samples with different concentrations (500, 100, 10, 1 microg/ml) of LBG as LBG groups. Blank control group in the absence of Lycium Barbarum glycopeptide or ConA and positive control group in the presence of 0.5 ml ConA but in the absence of LBG were created. 0.5 ml LBG samples with different concentrations in combination with 0. 5 ml ConA (10 microg/ml) into each well to observe the synergic effects of LBG and ConA as LBG+ConA groups. After incubation for 72 h at 37 degrees C, the samples were analyzed by CFSE-labeled cells combined with flow cytometry, and MTT. Flow cytometry revealed that both LBG could enhance the murine splenic lymphocyte proliferative reaction. Combined use of LBG and ConA had synergic effects. MTT demonstrated that sample A could obviously promote the murine splenic lymphocyte proliferative reaction as compared with control group (P<0. 01), while sample B could also enhance the lymphocyte proliferation at a high dose. In combination with ConA, sample A had synergic effects at high dose, while sample B showed obviously synergic effects (P<0.05). It was concluded that both samples (A and B) had strong immunocompetence.
PMID: 15641709 [PubMed - indexed for MEDLINE]
78: Biocell. 2004 Dec;28(3):271-7.
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Microcutting culture and morpho-physiological changes during acclimation in two Lycium chilense cytotypes.
Maseda PH, Lemcoff JH, Murúa M, Frayssinet N, Carceller MS.
IFEVA--Cátedra de Fisiología Vegetal (CONICET-FAUBA), Universidad de Buenos Aires. Av. San Martin 4453, Buenos Aires, Argentina. maseda@ifeva.edu.ar
Lycium chilense, a deciduous perennial shrub, is one of the endangered native species of Patagonia due to sheep overgrazing. Chances of recolonization by seeds are scarce due to the limited density of propagules in the soil and very specific requirements for germination. The objective was to develop an in vitro propagation protocol that would help to perform reestablishment of this species in degraded areas of the Patagonian steppe. Seeds came from two provenances with different somatic number due to differences in ploidy level. Defoliated microcuttings were planted in test tubes with different growing media and taken to a growth chamber. Rooting percentage did not differ between origins, but higher values were encountered for medium without hormones. Subcultures increased significantly rooting percentage and reduced time to rooting. The leaves from micropropagated plants were thinner, did not exhibit hairs, and had poorly developed palisade parenchyma and less epicuticular waxes. In vitro leaves had lower stomatal density and their stomata were less functional when compared to acclimated leaves. A repopulation program of Lycium chilense based on microcutting culture, specially using subcultures, is feasible.
Publication Types:
PMID: 15633450 [PubMed - indexed for MEDLINE]
79: J Ethnopharmacol. 2005 Jan 15;96(3):529-35. Epub 2004 Dec 8.
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Protective effect of Lycium chinense fruit on carbon tetrachloride-induced hepatotoxicity.
Ha KT, Yoon SJ, Choi DY, Kim DW, Kim JK, Kim CH.
Department of Pathology, Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University, 707 Suk-Jang Dong, Kyungju City, Kyungbuk 780-714, Korea.
In the present study, we investigated the protective effect of Lycium chinense Miller (Solanaceae) fruit (LFE) against CCl(4)-induced hepatotoxicity and the mechanism underlying these protective effects in rats. The pretreatment of LFE has shown to possess a significant protective effect by lowering the serum aspartate and alanine aminotransferase (AST and ALT) and alkaline phosphatase (ALP). This hepatoprotective action was confirmed by histological observation. In addition, pretreatment of LFE prevented the elevation of hepatic malondialdehyde (MDA) formation and the depletion of reduced glutathione (GSH) content and catalase activity in the liver of CCl(4)-injected rats. The LFE also displayed hydroxide radical scavenging activity in a dose-dependent manner (IC(50) = 83.6 microg/ml), as assayed by electron spin resonance (ESR) spin-trapping technique. The expression level of cytochrome P450 2E1 (CYP2E1) mRNA and protein, as measured by reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot analysis, was significantly decreased in the liver of LFE-pretreated rats when compared with that in the liver of control group. Based on these results, it was suggested that the hepatoprotective effects of the LFE might be related to antioxidative activity and expressional regulation of CYP2E1.
Publication Types:
PMID: 15619574 [PubMed - indexed for MEDLINE]
80: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao.
2004 Jun;30(3):261-8.
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[Role of exogenous Ca2+ in the somatic embryogenesis of Lycium barbarum L.]
[Article in Chinese]
Xing GM, Jing RF, Li S, Zhang X, Xu HX, Cui KR, Yu CH, Wang YF.
Institute of High Energy Physical, Chinese Academy of Sciences, Beijing 100039, China. xinggm@ihep.ac.cn
In this study, Embryogenic and non-embryogenic calli were separately obtained by cultivation of leaf segments on MS medium containing and not containing 2,4-D 0.2 mg/L. The calli were transferred to an 2,4-D-free MS medium containing different concentrations of (45)Ca(2+) and EGTA cultured, and microscopic examination of tissue sliced, gamma-ray energy spectrum analysis, ELISA and two-dimensional polyacrylamide-gel electrophoresis were used to study the relation between changes in Ca(2+) concentration and protein composition changes during somatic embryogenesis. The results showed that: (1) Calli of dedifferentiation were obtained by cultivating in the inductive medium (MS+2,4-D 0.2 mg/L) and then transferred to the 2,4-D-free (MS) differentiation medium. After cultivating, the large number of embryogenic cells divided and somatic embryogenic calli (EC) were formed; embryogenic cell differentiation and somatic embryo ware not formed when the dedifferentiation calli, which were cultivated in the inductive medium without 2,4-D, ware transferred to the cultivating of differentiation, so calli were called non-embryogenic calli (NEC). (2) SE frequency of EC was rised with exogenous Ca(2+) concentration was going up, and adding peak value (70.5% to 74.5%) when Ca(2+)concentration was from 0.8 to 1.6 mmol/L, then SE frequency was dropped markedly with Ca(2+) concentration was farther increasing. Formation of meristematic cell aggregates of NEC was also enhanced when exogenous Ca(2+) concentration was from 0.8 to 1.6 mmol/L. (3) After adding EGTA, which was Ca(2+) antagonist, SE frequency was dropped markedly, and SE frequency was fallen along with increased of EGTA concentration. When EGTA concentration went up to 1.2 micromol/L, SE frequency dropped to 5%, and the formation of meristematic cell aggregates on NEC was inhibited. (4) When 2 microCi (45)Ca(2+)/mL was added, the uptake of (45)Ca(2+) by EC and NEC was different, two uptake peaks of (45)Ca(2+)appeared in EC at the embryogenic cell differentiation of stage, and the uptake of (45)Ca(2+) of EC was 4-5 times higher than that of NEC, and the uptake frequency of (45)Ca(2+) improved from 54.1% to 74.5%. The uptake of (45)Ca(2+) by NEC during development not only was lower than that by EC but also there were no such marked peak as those with EC. (5) The CaM content examined by ELISA was increased markedly at multi-cellular embryo and globular embryoid stage of EC. After adding Ca(2+), the CaM content increased significantly, the CaM content of EC was 2-3 times that of NEC. (6) The IEF/SDS-PAGE results showed that the numbers and amount of protein components were widely different between the two kinds of callus with different morphogenesis patterns, the number of proteins of EC had more components than those of NEC. The largest differences protein species presented with Ca(2+) ware added, the more proteins presented on the range of molecular weight was from 43 kD to 66 kD and pI values was from 4.0 to 7.0.
Publication Types:
PMID: 15599021 [PubMed - indexed for MEDLINE]
81: Life Sci. 2004 Nov 26;76(2):137-49.
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Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum.
Luo Q, Cai Y, Yan J, Sun M, Corke H.
College of Public Health, Wuhan University, Wuhan 430071, Hubei, PR China.
The hypoglycemic and hypolipidemic effects of Lycium barbarum fruit water decoction, crude polysaccharide extracts (crude LBP), and purified polysaccharide fractions (LBP-X) in alloxan-induced diabetic or hyperlipidemic rabbits were investigated through designed sequential trials and by measuring blood glucose and serum lipid parameters. Total antioxidant capacity was also assessed using trolox equivalent antioxidant capacity (TEAC) and oxygen radical absorbance capacity (ORAC) assay. It was found that the three Lycium barbarum fruit extracts/fractions could significantly reduce blood glucose levels and serum total cholesterol (TC) and triglyceride (TG) concentrations and at same time markedly increase high density lipoprotein cholesterol (HDL-c) levels after 10 days treatment in tested rabbits, indicating that there were substantial hypoglycemic and hypolipidemic effects. Hypoglycemic effect of LBP-X was more significant than those of water decoction and crude LBP, but its hypolipidemic effect seemed to be weaker. Total antioxidant capacity assay showed that all three Lycium barbarum extracts/fractions possessed antioxidant activity. However, water and methanolc fruit extracts and crude polysaccharide extracts exhibited stronger antioxidant activity than purified polysaccharide fractions because crude extracts were identified to be rich in antioxidants (e.g., carotenoids, riboflavin, ascorbic acid, thiamine, nicotinic acid). Lycium barbarum polysaccharides (glycocojugates), containing several monosaccharides and 17 amino acids, were major bioactive constituents of hypoglycemic effect. Both polysaccharides and vitamin antioxidants from Lycium barbarum fruits were possible active principles of hypolipidemic effect.
Publication Types:
PMID: 15519360 [PubMed - indexed for MEDLINE]
82: Ying Yong Sheng Tai Xue Bao. 2004 Apr;15(4):549-55.
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[Niche characteristics of plants on four environmental gradients in middle reaches of Tarim River]
[Article in Chinese]
Liu J, Chen Y, Zhang Y.
Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. kljkzx@sohu.com
Tarim River is the longest continental river in China. Along its either bank, the natural vegetation belts are composed of arbors, shrubs and grasses. In this paper, the niche breadths and overlaps of nineteen species on four environmental gradients in the middle reaches of Tarim River were calculated and analyzed. The results showed that the nineteen species could be divided into three groups, based on their average niche breadths. Group one included Populus euphratica, Tamarix ramosissima, T. hispida, Lycium ruthernicum, Alhagi sparsifolia and Phragmites australis, which were constructive species in Tarim River and their niche breadths were greater. Group two included T. leptostachys, Aeluropus pungens, Poacynum hendersonii, Cynanchum sibiricum, Karelinia caspica, Inula salsoloides, Taraxacum sp., and Hexinia polydichotoma, and their niche breadths were smaller than that of group one. Group three included Halimodendron halodendron, Glycyrrhiza inflata, Calamagrostis pseudophragmites, Salsola sp., and Sophora alapecuroides. Based on the niche overlaps matrix, the niche overlaps of the plant species pairs were generally smaller on all the four environmental gradients, because of their probable niche shift or the demand for different environmental resources. Soil moisture and soil salinity were the important environmental factors limiting the plant distribution in the middle reaches of Tarim River.
Publication Types:
PMID: 15334943 [PubMed - indexed for MEDLINE]
83: Biotechnol Lett. 2004 Jul;26(14):1125-30.
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Anti-fungal effects of phenolic amides isolated from the root bark of Lycium chinense.
Lee DG, Park Y, Kim MR, Jung HJ, Seu YB, Hahm KS, Woo ER.
Department of Microbiology, Kyungpook National University, Daegu 702-701, Republic of Korea.
Four phenolic amides, dihydro-N-caffeoyltyramine (1), trans-N-feruloyloctopamine (2), trans-N -caffeoyltyramine (3), and cis-N-caffeoyltyramine (4), were isolated from an ethyl acetate extract of the root bark of Lycium chinense Miller. All had an anti-fungal effect; compounds 1-3 were potent at 5-10 microg ml(-1) and were without hemolytic activity against human erythrocyte cells. Compound 4 was active at 40 microg ml(-1). All four compounds impeded the dimorphic transition of pathogen, Candida albicans.
Publication Types:
PMID: 15266117 [PubMed - indexed for MEDLINE]
84: Phytother Res. 2004 Apr;18(4):301-5.
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Effects of betaine, coumarin and flavonoids on mucin release from cultured hamster tracheal surface epithelial cells.
Lee CJ, Lee JH, Seok JH, Hur GM, Park Js J, Bae S, Lim JH, Park YC.
Department of Pharmacology, College of Medicine, Chungnam National University, Daejeon, Korea. LCJ123@cnu.ac.kr
Betaine, coumarin, hesperidin and kaempferol are the components derived from Lycium chinense, Angelicae decursiva, Poncirus trifoliata and Polygonatum odoratum, respectively. These plants have been used for the treatment of respiratory diseases in oriental medicine and their respective components were reported to have various biological effects. In this study, we investigated whether these natural products affect mucin release from cultured hamster tracheal surface epithelial cells and compared the possible activities of these agents with the inhibitory action on mucin release by poly-L-lysine and the stimulatory action by adenosine triphosphate. Confluent primary hamster tracheal surface epithelial cells were metabolically radiolabeled using (3)H-glucosamine for 24 h and treated for 30 min in the presence of varying concentrations of each agent to assess the effects on (3)H-mucin release. The results were as follows: (i) Coumarin and kaempferol did not affect mucin release significantly; (ii) Betaine and hesperidin increased mucin release at the highest concentration; (iii) Poly-L-lysine inhibited and adenosine triphosphate increased mucin release. We conclude that betaine and hesperidin can increase mucin release by direct acting on airway mucin-secreting cells and suggest these agents be further studied for the possible use as mild expectorants during the treatment of chronic airway diseases. Copyright 2004 John Wiley & Sons, Ltd.
PMID: 15162365 [PubMed - indexed for MEDLINE]
85: J Agric Food Chem. 2004 Jun 2;52(11):3536-41.
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Identification of monomenthyl succinate, monomenthyl glutarate, and dimenthyl glutarate in nature by high performance liquid chromatography-tandem mass spectrometry.
Hiserodt RD, Adedeji J, John TV, Dewis ML.
International Flavors & Fragrances Inc (IFF), Research and Development, 1515 State Highway 36, Union Beach, New Jersey 07735, USA. richard.hiserodt@iff.com
Menthol, menthone, and other natural compounds provide a cooling effect and a minty flavor and have found wide application in chewing gum and oral care products. Monomenthyl succinate, monomenthyl glutarate, and dimenthyl glutarate provide a cooling effect without the burning sensation associated with menthol. Additionally, because they do not have a distinct flavor, they can be used in applications other than mint flavors. Because these menthyl esters have not been reported in nature, we undertook to identify a natural source for these cooling compounds. Using high performance liquid chromatography-tandem mass spectrometry, monomenthyl succinate was identified in Lycium barbarum and Mentha piperita, and monomenthyl glutarate and dimenthyl glutarate were identified in Litchi chinesis. The identifications were based on the correlation of mass spectrometric and chromatographic retention time data for the menthyl esters in the extracts with authentic standards which resulted in a 99.980% confidence in the identifications.
PMID: 15161227 [PubMed - indexed for MEDLINE]
86: Br J Nutr. 2004 May;91(5):707-13.
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Comparison of plasma responses in human subjects after the ingestion of 3R,3R'-zeaxanthin dipalmitate from wolfberry (Lycium barbarum) and non-esterified 3R,3R'-zeaxanthin using chiral high-performance liquid chromatography.
Breithaupt DE, Weller P, Wolters M, Hahn A.
Institute for Food Chemistry, University of Hohenheim, Garbenstrasse 28, 70593 Stuttgart, Germany. breithau@uni-hohenheim.de
Age-related macular degeneration (AMD) is one of the most common eye diseases of elderly individuals. It has been suggested that lutein and zeaxanthin may reduce the risk for AMD. Information concerning the absorption of non-esterified or esterified zeaxanthin is rather scarce. Furthermore, the formation pathway of meso (3R,3'S)-zeaxanthin, which does not occur in plants but is found in the macula, has not yet been identified. Thus, the present study was designed to assess the concentration of 3R,3R'-zeaxanthin reached in plasma after the consumption of a single dose of native 3R,3'R-zeaxanthin palmitate from wolfberry (Lycium barbarum) or non-esterified 3R,3'R-zeaxanthin in equal amounts. In a randomised, single-blind cross-over study, twelve volunteers were administered non-esterified or esterified 3R,3'R-zeaxanthin (5 mg) suspended in yoghurt together with a balanced breakfast. Between the two intervention days, a 3-week depletion period was inserted. After fasting overnight, blood was collected before the dose (0 h), and at 3, 6, 9, 12, and 24 h after the dose. The concentration of non-esterified 3R,3'R-zeaxanthin was determined by chiral HPLC. For the first time, chiral liquid chromatography-atmospheric pressure chemical ionisation-MS was used to confirm the appearance of 3R,3'R-zeaxanthin in pooled plasma samples. Independent of the consumed diet, plasma 3R,3'R-zeaxanthin concentrations increased significantly (P=0.05) and peaked after 9-24 h. Although the concentration curves were not distinguishable, the respective areas under the curve were distinguishable according to a two-sided F and t test (P=0.05). Thus, the study indicates an enhanced bioavailability of 3R,3'R-zeaxanthin dipalmitate compared with the non-esterified form. The formation of meso-zeaxanthin was not observed during the time period studied.
Publication Types:
PMID: 15137922 [PubMed - indexed for MEDLINE]
87: Int Immunopharmacol. 2004 Apr;4(4):563-9.
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Immunomodulation and antitumor activity by a polysaccharide-protein complex from Lycium barbarum.
Gan L, Hua Zhang S, Liang Yang X, Bi Xu H.
Institute of Materia Medica, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China. ganlu07@163.com
The modulation of a polysaccharide-protein complex from Lycium barbarum (LBP3p) on the immune system in S180-bearing mice was investigated. The mice inoculated with S180 cell suspension were treated p.o. with LBP3p (5, 10 and 20 mg/kg) for 10 days. The effects of LBP3p on transplantable tumors and macrophage phagocytosis, quantitative hemolysis of sheep red blood cells (QHS), lymphocyte proliferation, the activity of cytotoxic T lymphocyte (CTL), interleukin-2 (IL-2) gene expression and lipid peroxidation were studied. LBP3p could significantly inhibit the growth of transplantable sarcoma S180 and increase macrophage phagocytosis, the form of antibody secreted by spleen cells, spleen lymphocyte proliferation, CTL activity, IL-2 mRNA expression level and reduce the lipid peroxidation in S180-bearing mice. The effect is not dose-dependent in a linear fashion. A total of 10 mg/kg dose is more effective than 5 and 20 mg/kg doses. This suggests that LBP3p at 10 mg/kg has a highly significant effect on tumor weight and improves the immune system. Copyright 2004 Elsevier B.V.
PMID: 15099534 [PubMed - indexed for MEDLINE]
88: Biol Pharm Bull. 2004 Apr;27(4):583-6.
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Antiosteoporotic activity of the water extract of Dioscorea spongiosa.
Yin J, Tezuka Y, Kouda K, Tran QL, Miyahara T, Chen Y, Kadota S.
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
After 60 MeOH and water extracts of natural crude drugs were screened for their ability to stimulate osteoblast proliferation, four MeOH extracts (Cynomorium songaricum, Drynaria fortunei, Lycium chinense, Rehmannia glutinosa) and seven water extracts (Cornus officinalis, Dendrobium nobile, Dioscorea spongiosa, Drynaria fortunei, Eucommia ulmoides, Lycium chinensis, Viscum coloratum) showed that potent activities were evaluated for inhibition of osteoclast formation. The results indicated that the water extract of D. spongiosa not only showed the strongest stimulation of osteoblast proliferation but also possessed potent inhibitory activity aganist osteoclast formation, whereas it showed lower cytotoxicity in osteoblast and bone marrow cells. A further in vivo experiment determined the antiosteoporotic activity of this extract, in which it inhibited the decrease in cancellous bone mineral content, cancellous bone mineral density, and cortical bone mineral content of the proximal tibia in ovariectomized rats.
Publication Types:
PMID: 15056872 [PubMed - indexed for MEDLINE]
89: J Agric Food Chem. 2004 Apr 7;52(7):2092-6.
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2-O-(beta-D-Glucopyranosyl)ascorbic acid, a novel ascorbic acid analogue isolated from Lycium fruit.
Toyoda-Ono Y, Maeda M, Nakao M, Yoshimura M, Sugiura-Tomimori N, Fukami H.
Health Care Science Laboratory, Institute for Food & Beverage, Technological Development Center, Suntory Ltd., 5-2-5, Yamazaki, Shimamoto-cho, Mishima-gun, Osaka 618-0001, Japan.
A novel stable precursor of ascorbic acid (vitamin C), 2-O-(beta-D-glucopyranosyl)ascorbic acid, was isolated from both the ripe fresh fruit and dried fruit of Lycium barbarum L., a plant of the Solanaceae family. The chemical structure was inferred by instrumental analyses and confirmed by chemical synthesis. The dried fruit of Lycium barbarum L. contained ca. 0.5% of it, which is comparable to the ascorbic acid content of fresh lemons. It increased the blood ascorbic acid by oral administration to rats, and it was also detected in blood from the portal vein.
PMID: 15053557 [PubMed - indexed for MEDLINE]
90: Zhongguo Zhong Yao Za Zhi. 2003 Aug;28(8):766-9.
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[Effects of the extract from bergamot and boxthorn on the delay of skin aging and hair growth in mice]
[Article in Chinese]
Shao LX.
College of Life and Environment Science, Zhejiang Normal University, Jinhua 321004, Zhejiang, China. shaolinxiang@sina.com
OBJECTIVE: To study the effects of the extract from bergamot and boxthorn on the skin and the hair growth in mice. METHOD: The skin on the back of mice was shaved topically and smeared with bergamot and boxthorn extract for 42 days. Then the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) and collagen in the mice were measured. The growth of hair was also observed. RESULT: Compared with control group, the extract from bergamot and boxthorn obviously increased the activity of superoxide dismutase (P < 0.05) and the content of collagen (P < 0.001), and decreased the content of malondialdehyde (P < 0.05) in the skin of mice. It also significantly promoted the growth of hair (P < 0.001). CONCLUSION: The extract from bergamot and boxthorn plays an active role in skin and the promotion of hair growth.
Publication Types:
PMID: 15015364 [PubMed - indexed for MEDLINE]
91: Zhongguo Zhong Yao Za Zhi. 2003 Aug;28(8):751-4.
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[Effects of one Chinese herbs on improving cognitive function and memory of Alzheimer's disease mouse models]
[Article in Chinese]
Sun H, Hu Y, Zhang JM, Li SY, He W.
Peking Union Medical College Hospital, CAMS and PUMC, Beijing 100730, China.
OBJECTIVE: To investigate Chinese herbs with the effect on significantly improving the cognitive function and retention of patients with Alzheimer's disease. METHOD: AD mouse models were established by injecting poly-beta-AP25-35 into the cerebral ventricle of mice. The curative effects of traditional Chinese herbs Dangguishaoyaosan, Chaihujialonggumulitang and two herbs (CHP I and CHP II) developed by the authors on improving the memory and cognitive function of AD mouse models were studied by the detection of behavioral and histochemical tests, with piracetam serving as control. RESULT: CHP II has profound curative effects on improving the memory and cognitive function of AD-like animal model. CONCLUSION: The present study indicates that it has a satisfactory prospect to seek new drugs from Chinese herbs to treat AD.
Publication Types:
PMID: 15015359 [PubMed - indexed for MEDLINE]
92: Wei Sheng Yan Jiu. 2003 Nov;32(6):599-601.
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[Study on protective action of lycium barbarum polysaccharides on DNA imparments of testicle cells in mice]
[Article in Chinese]
Huang X, Yang M, Wu X, Yan J.
School of Public Health, Wuhan University, Wuhan 430071, China.
To investigate the protective effect of lycium barbarum polysaccharides (LBP) on DNA oxidative damage of testicle cells induced by hydrogen peroxide (H2O2). The single cell gel electrophoresis(SCGE) was used to detect the breakage of DNA strand and analyze LBP protection against oxidation damage in testicle cells treated by different concentrations of LBP for 1 hour firstly, and then cultured with 100 mumol/L H2O2 for 25 min. The results showed that a certain moment action by H2O2 could induce the breakage of DNA strand. The pretreatment of LBP (50, 100, 200, 400 micrograms/ml) significantly decreased the frequencies of cells with tail moment and the tail length of testicle cells treated by H2O2. It is suggested that LBP itself could not cause the oxidation damage, but it could clean out the free-radical and restrain the DNA damage of testicle cells caused by the oxidative stress.
Publication Types:
PMID: 14963914 [PubMed - indexed for MEDLINE]
93: Shi Yan Sheng Wu Xue Bao. 2003 Dec;36(6):414-20.
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[Ultracytochemical localization of calcium and ATPase activity on the 2,4-D induced somatic embryogenesis of Lycium barbarum L.]
[Article in Chinese]
Li S, Xing GM, Cui KR, Yu CH, Zhang X, Xu HX, Wang YF.
College of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030.
In order to research the function mechanism of the 2,4-D during the development of plant somatic embryogenesis, we studied its function mechanism and relationship with the space-time distributing of Ca2+ content and ATPase activity on somatic embryogenesis of Lycium barbarum L. The possible effects on 2,4-dichlorophenoxyacetic acid (2,4-D) induced somatic embryogenesis and changes of Ca2+ and ATPase active at different development period of somatic embryogenesis. The result showed: The 2,4-D was a key hormone for induced embryonic state of Lycium barbarum L. The embryonic callus and non-embryonic callus was separately obtained in the medium that contains the auxin 2,4-D and lack 2,4-D. In the present study, we have observed the Ca2+ was more abundant in the further intercellular matrix and on the cell wall at the multi-cellular stage, and Ca2+ was concentrated in the plasma membrane and vacuoles membrane during embryonic cell differentiate and division, to the globular embryo, more Ca2+ was seen in the nucleus. Afterward, it was also observed to be distributed in the thicken cell wall and intercellular matrix. At the same process, the variations of ATPase activity and Ca2+ were highly similar, ATPase activity was mainly located on the plasma membrane in early embryogenic cells. With further development, it was also observed to be distributed in endoplasm, nucleus and vacuoles, with the thickening of embryogenic cell wall, ATPase activity was found in the thickened region and the intercellular space. However, the variations of ATPase activity and Ca2+ have not clearly observed variety dynamics at the nonembryogenic callus, and with further vacuolation of nonembryogenic cell, Ca2+ content and ATPase activity gradually drop. It was indicated there was a closely relationship between the dynamics of Ca2+ and ATPase activity in somatic embryogenesis by 2,4-D induced. And the space-time distribution of Ca2+ and ATPase activity play a key role on signal transmission and the regulation of relevant gene expression.
Publication Types:
PMID: 14724931 [PubMed - indexed for MEDLINE]
94: Zhongguo Zhong Xi Yi Jie He Za Zhi. 2003 Dec;23(12):926-8.
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[Effect of wolfberry fruit and epimedium on DNA synthesis of the aging-youth 2BS fusion cells]
[Article in Chinese]
Wu BY, Zou JH, Meng SC.
Department of Cell Biology and Genetics, Medical College, Peking University, Beijing 100083. baiyanwu@public.fhnet.cn.net
OBJECTIVE: To study the effect of water extracts of Wolfberry fruit (WB) and Epimedium (EM) on DNA synthesis of the aging-youth 2BS fusion cells. METHODS: Human embryonic lung diploid fibroblasts 2BS national standard strain, were used as an aging model. Cell denucleation and cell fusion techniques were applied to observe the effect of WB and EM on DNA synthesis of 2BS fusion cells. RESULTS: In the 0.025 (V/V) WB or EM water extract containing media, 2BS cells could be continuously cultured for 61.0 +/- 2.9 passages and 56.0 +/- 2.6 passages respectively, while in the control group it was only 49.0 +/- 2.6 passages (P < 0.01). After treatment with WB and EM separately for 2 hrs, the aging 2BS cells were denucleated and fused with young 2BS cells. The [3H]TdR incorporation percentage in these treated cells was significantly higher than that in the untreated control cells (P < 0.01). CONCLUSION: Both WB and EM can accelerate the DNA synthesis rate of the aging youth 2BS fusion cells and prolong the life span of 2BS cells.
Publication Types:
PMID: 14714367 [PubMed - indexed for MEDLINE]
95: Phytomedicine. 2003 Nov;10(8):650-6.
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Inhibition of monoamine oxidase B (MAO-B) by Chinese herbal medicines.
Lin RD, Hou WC, Yen KY, Lee MH.
Department of Internal Medicine, Municipal Taipei Ho-Ping Hospital, Taiwan.
Monoamine oxidase (MAO) catalyzes the oxidative deamination of biogenic amines accompaned by the release of H2O2. Two subtypes, MAO-A and MAO-B, exist on the basis of their specificities to substrates and inhibitors. The regulation of MAO-B activity is important in the treatment of neurodegenerative diseases. Twenty-seven species of plants used in traditional Chinese medicines, selected from an enthnobotanical survey, were used in an investigation of their inhibitory effect on MAO-B in rat brain homogenates. The 50% aqueous methanol extracts of four active extracts, Arisaema amurense, Lilium brownii var. colchesteri, Lycium chinense, and Uncaria rhynchophylla, exhibited the best activity and selectivity towards MAO-B with IC50 values of 0.44, 0.29, 0.40, and 0.03 mg/ml, respectively. A kinetic study of MAO-B inhibition by the four extracts using the Lineweaver-Burk plot for each active extract revealed the IC50 concentrations, and results show that: Ki = 0.59 mg/ml for A. amurense for the mixed-type mode, Ki = 0.58 mg/ml for L. brownii var. colchesteri for the mixed-type mode, Ki = 5.01 mg/ml for L. chinense for the uncompetitive mode, and Ki = 0.02 mg/ml for U. rhynchophylla for the uncompetitive mode. These may therefore be candidates for use in delaying the progressive degeneration caused by neurological diseases.
Publication Types:
PMID: 14692725 [PubMed - indexed for MEDLINE]
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